Crystallization of proteins from polyethylene glycol.
نویسندگان
چکیده
منابع مشابه
Sensitive measurement of polyethylene glycol-modified proteins.
An IgM monoclonal antibody (AGP3) against polyethylene glycol (PEG) was used to assay PEG-modified proteins by ELISA. PEG-modified beta-glucuronidase could be measured at concentrations as low as 15 ng/mL, corresponding to 750 pg (1.8 fmol) of conjugate. This ELISA should be generally applicable to all PEG-modified proteins because AGP3 binds the backbone of the PEG chain independent of the lin...
متن کاملCrystallization of yeast triose phosphate isomerase from polyethylene glycol. Protein crystal formation following phase separation.
A new crystal form of yeast triose phosphate isomerase has been grown from solutions of the enzyme in polyethylene glycol (PEG) of average molecular weight 4000. The crystals are monoclinic, space group P21, with a = 61.3 A, b = 98.4 A, c = 49.7 A, /3 = 90.9”. There is one dimeric molecule per asymmetric unit. Data are observable to at least 1.3A resolution. Crystallization can be achieved afte...
متن کاملStability of Trinitroglycerin in Polyethylene Glycol 400
In this study, the stability of trinitroglycerin (TNG) in polyethylene (PEG) 400 was investigated according to Arrhenius approach. The reaction rate constant (k) for the thermal disintegration of TNG in PEG 400 at various temperatures was counted based on the percents of remaining TNG at different intervals of time at a specific temperature. The results showed that “k” at 40, 50, 60, 70, 8...
متن کاملImmunogenicity of Polyethylene Glycol (PEG)
Covalent attachment of polyethylene glycol (PEG) to active proteins (PEGylation technology) successfully generated several FDA-approved compounds, including four blockbusters, which are considered non immunogenic. However, PEG antibodies and intolerance to the infusion were reported in some patients with gout treated with pegloticase, a PEG-uricase recently approved by the FDA (Food and Drug Ad...
متن کاملWhat proteins are present in polyethylene glycol precipitates from rheumatic sera?
The proteins present in 4% polyethylene glycol (PEG) precipitates of 10 normal sera and 60 samples from patients with rheumatic diseases were studied. A variety of immunochemical methods were used, including estimation of the percentages of total serum proteins precipitated by PEG, gel filtration analyses of the precipitates, and affinity chromatography with protein A and anti-immunoglobulin co...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1976
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(20)81858-4